2023
Galley N, Greetham D, Alaman-Zarate M, Williamson M, Evans C, Spittal W, Buddle J, Freeman J, Davis G, Dickman M, Wilcox M, Lovering A, Fagan R, Mesnage S
Clostridioides difficile canonical L,D-transpeptidases catalyse a novel type of peptidoglycan crosslinks and are not required for beta-lactam resistance.
The Journal of biological Chemistry, in press, DOI:https://doi.org/10.1016/j.jbc.2023.105529
Alaman-Zarate M*, Rady J*, Evans C, Pian B, Greetham D, Mareco-Ortiz S, Dickman M, Lidbury I, Lovering A, Barstow B, Mesnage S
Unusual 1-3 peptidoglycan cross-links in Acetobacteraceae are made by L,D-transpeptidases with a catalytic domain distantly related to YkuD domains.
The Journal of biological Chemistry, in press, 10.1016/j.jbc.2023.105494
Salamaga B, Turner R, Elsarmane F, Galley N, Kulakauskas S, Mesnage S
A moonlighting role for LysM peptidoglycan binding domains underpidoi: 10.1038/s42003-023-04808-zns Enterococcus faecalis daughter cel separation.
Commun Biol, 6(1):428. doi: 10.1038/s42003-023-04808-z
2022
Davis JL, Hounslow AM, Baxter NJ, Mesnage S, Williamson MP
1H, 13C and 15N resonance assignments of a conserved putative cell wall binding domain from Enterococcus faecalis.
Biomolecular NMR assignments, doi.org/10.1007/s12104-022-10087-2
Ledger EVK, Mesnage S, Edwards AM
Human serum triggers antibiotic tolerance in Staphylococcus aureus.
Nature comms,13, 2041. doi.org/10.1038/s41467-022-29717-3
Zamboni V, Barelier S, Dixon R, Galley NF, Ghanem A, Nguyen QP, Cahuzac H, Salamaga B, Davis PJ, Bourne Y, Mesnage S*, Vincent F*
Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis.
The Journal of Biological Chemistry, 7:101915. doi: 10.1016/j.jbc.2022.101915
2021
Patel AP, Turner RD, Rifflet A, Acosta-Martin AE, Nichols A, Awad MM, Lyras D, Gomperts-Boneca I, Bern M, Collins MO, Mesnage S.
A novel pipeline for the consistent, reproducible and high-resolution structural analysis of bacterial peptidoglycans.
Elife, 10:e70597. DOI: 10.7554/eLife.70597.
Małecki PH, Mitkowski P, Jagielska E, Trochimiak K, Mesnage S, Sabała I.
Structural characterization of EnpA D,L-endopeptidase from Enterococcus faecalis prophage provides insights into substrate specificity
of M23 peptidases.
Int J Mol Sci. 22(13):7136. doi: 10.3390/ijms22137136.
2020
Galley N, Greetham D, Alaman-Zarate M, Williamson M, Evans C, Spittal W, Buddle J, Freeman J, Davis G, Dickman M, Wilcox M, Lovering A, Fagan R, Mesnage S
Clostridioides difficile canonical L,D-transpeptidases catalyse a novel type of peptidoglycan crosslinks and are not required for beta-lactam resistance.
The Journal of biological Chemistry, in press, DOI:https://doi.org/10.1016/j.jbc.2023.105529
Alaman-Zarate M*, Rady J*, Evans C, Pian B, Greetham D, Mareco-Ortiz S, Dickman M, Lidbury I, Lovering A, Barstow B, Mesnage S
Unusual 1-3 peptidoglycan cross-links in Acetobacteraceae are made by L,D-transpeptidases with a catalytic domain distantly related to YkuD domains.
The Journal of biological Chemistry, in press, 10.1016/j.jbc.2023.105494
Salamaga B, Turner R, Elsarmane F, Galley N, Kulakauskas S, Mesnage S
A moonlighting role for LysM peptidoglycan binding domains underpidoi: 10.1038/s42003-023-04808-zns Enterococcus faecalis daughter cel separation.
Commun Biol, 6(1):428. doi: 10.1038/s42003-023-04808-z
2022
Davis JL, Hounslow AM, Baxter NJ, Mesnage S, Williamson MP
1H, 13C and 15N resonance assignments of a conserved putative cell wall binding domain from Enterococcus faecalis.
Biomolecular NMR assignments, doi.org/10.1007/s12104-022-10087-2
Ledger EVK, Mesnage S, Edwards AM
Human serum triggers antibiotic tolerance in Staphylococcus aureus.
Nature comms,13, 2041. doi.org/10.1038/s41467-022-29717-3
Zamboni V, Barelier S, Dixon R, Galley NF, Ghanem A, Nguyen QP, Cahuzac H, Salamaga B, Davis PJ, Bourne Y, Mesnage S*, Vincent F*
Molecular basis for substrate recognition and septum cleavage by AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis.
The Journal of Biological Chemistry, 7:101915. doi: 10.1016/j.jbc.2022.101915
2021
Patel AP, Turner RD, Rifflet A, Acosta-Martin AE, Nichols A, Awad MM, Lyras D, Gomperts-Boneca I, Bern M, Collins MO, Mesnage S.
A novel pipeline for the consistent, reproducible and high-resolution structural analysis of bacterial peptidoglycans.
Elife, 10:e70597. DOI: 10.7554/eLife.70597.
Małecki PH, Mitkowski P, Jagielska E, Trochimiak K, Mesnage S, Sabała I.
Structural characterization of EnpA D,L-endopeptidase from Enterococcus faecalis prophage provides insights into substrate specificity
of M23 peptidases.
Int J Mol Sci. 22(13):7136. doi: 10.3390/ijms22137136.
2020
- Sandoz KM, Moore RA, Beare PA, Patel AV, Smith RE, Bern M, Hwang H, Cooper CJ, Priola SA, Parks JM, Gumbart JC, Mesnage S*, Heinzen RA*.
- β-Barrel proteins tether the outer membrane in many Gram-negative bacteria.
- Nature Microbiology, doi: 10.1038/s41564-020-00798-4.
- Sibinelli-Sousa S, Hespanhol JT, Nicastro GG, Matsuyama BY, Mesnage S, Patel A, de Souza RF, Guzzo CR, Bayer-Santos E.
- A Family of T6SS antibacterial effectors related to L,D-transpeptidases targets the peptidoglycan
- Cell Reports 31, 107813. doi.org/10.1016/j.celrep.2020.107813.
- Guerardel Y, Sadovskaya I, Maes E, Furlan S, Chapot-Chartier MP, Mesnage S, Rigottier-Gois L, Serror P.
- Complete structure of the Enterococcal Polysaccharide Antigen (EPA) of Vancomycin-Resistant Enterococcus faecalis V583 reveals that EPA
decorations are teichoic acids covalently linked to a rhamnopolysaccharide backbone. - mBio, 11(2):e00277-20. doi: 10.1128/mBio.00277-20.
- Gonzalez-Delgado LS, Waters-Morgan H, Salamaga B, Robertson AJ, Hounslow AM, Jagielska E, Sabala I, Williamson MP, Lovering AL, Mesnage S.
- Two site recognition of Staphylococcus aureus peptidoglycan by lysostaphin SH3b.
- Nature Chemical Biology, doi: 10.1038/s41589-019-0393-4.
- 2019
- Bublitz DC, Chadwick GL, Magyar JS, Sandoz KM, Brooks D, Mesnage S, Ladinsky MS, Garber AI, Bjorkman PJ, Orphan VJ, McCutcheon JP.
- Peptidoglycan production by an insect-bacterial Mosaic.
- Cell, 179(3):703-712.e7. doi: 10.1016/j.cell.2019.08.054
- Smith RE, Salamaga B, Szkuta P, Hajdamowicz N, Prajsnar TK, Bulmer GS, Fontaine T, Kołodziejczyk J, Herry JM, Hounslow AM, Williamson MP, Serror P, Mesnage S.
- Decoration of the enterococcal polysaccharide antigen EPA is essential for virulence, cell surface charge and interaction with effectors of the
innate immune system.
- Al-Zubidi M Widziolek M, Court E, Gains A, Smith RE, Ansbro K, Alrafaie A, Evans C, Murdoch C, Mesnage S, Douglas CW, Rawlinson A, Stafford GP.
Identification of novel bacteriophages with therapeutic potential that target Enterococcus faecalis. - Infection and Immunity, 2019, 87(11). doi: 10.1128/IAI.00512-19.
- 2018
- Lund VA, Wacnik K, Turner RD, Cotterell B, Walther C, Fenn SJ, Grein F, Wollman AJ, Leake MC, Olivier N, Cadby A, Mesnage S, Jones S, Foster SJ.
- Molecular coordination of Staphylococcus aureus cell division.
- Elife, 7: e32057 doi: 10.7554/eLife.32057.
- Turner RD, Mesnage S, Hobbs JK, Foster SJ.
- Molecular imaging of glycan chains couples cell-wall polysaccharide architecture to bacterial cell morphology.
- Nature Communications, 9: 1263. doi: 10.1038/s41467-018-03551-y.
- 2017
- Salamaga B, Prajsnar TK, Jareño-Martinez A, Willemse J, Bewley MA, Chau F, Ben Belkacem T, Meijer A, Dockrell D, Renshaw SA, Mesnage S.
- Bacterial size matters: multiple mechanisms controlling septum cleavage and diplococcus formation are critical for the virulence of the
opportunistic pathogen Enterococcus faecalis. - PLoS Pathogens, 13(7):e1006526. doi: 10.1371/journal.ppat.1006526.
- Zhang X, de Maat V, Guzmán Prieto AM, Prajsnar TK, Bayjanov J, de Been M, Rogers M, Bonten MJM, Mesnage S, Willems RJL, van Schaik W.
- RNA-seq and Tn-seq reveal fitness determinants of vancomycin-resistant Enterococcus faecium during growth in human serum.
- BMC Genomics, 18: 893. doi: 10.1186/s12864-017-4299-9.
-
- Mihelič M, Vlahoviček-Kahlina K, Renko M, Mesnage S, Doberšek A, Taler-Verčič A, Jakas A, Turk D.
- The mechanism behind the selection of two different cleavage sites in NAG-NAM polymers.
- IUCrJ, 4: 185-198. doi: 10.1107/S2052252517000367.
- 2016
- Bern M, Beniston R, Mesnage S.
- Towards an automated analysis of bacterial peptidoglycan Structure.
- Analytical and Bioanalytical Chemistry. 409: 551-560. doi: 10.1007/s00216-016-9857-5.
- Rodríguez-Rubio L, Gerstmans H, Thorpe S, Mesnage S, Lavigne R, Briers Y.
- DUF3380 domain from a Salmonella phage endolysin shows potent N-acetylmuramidase activity.
- Applied and Environmental Microbiology, 82: 4975-4981. doi: 10.1128/AEM.00446-16.
- Oliveira H, Vilas Boas D, Mesnage S, Kluskens LD, Lavigne R, Sillankorva S, Secundo F, Azeredo J.
- Structural and enzymatic characterization of ABgp46, a novel phage endolysin with broad anti-Gram-negative bacterial activity.
- Frontiers in Microbiology, 7: 208. doi: 10.3389/fmicb.2016.00208.
- 2015
- Wheeler R, Turner RD, Bailey RG, Salamaga B, Mesnage S, Mohamad SAS, Hayhurst EJ, Horsburgh M, Hobbs J, Foster SJ.
- Bacterial cell enlargement requires control of cell wall stiffness mediated by peptidoglycan hydrolases.
- mBio, 6(4):e00660-15. doi:10.1128/mBio.00660-15.
-
- Willing SE, Candela T, Shaw HA, Seager Z, Mesnage S, Fagan RP, Fairweather NF.
- Clostridium difficile surface proteins are anchored to the cell wall using CWB2 motifs that recognise the anionic polymer PSII.
- Molecular Microbiology, 96: 596-608. doi: 10.1111/mmi.12958
- 2014
- Mesnage S, Dellarole M, Baxter N, Rouget JB, Dimitrov J, Wang N, Fujimoto Y, Hounslow A, Lacroix-Desmazes S, Fukase K, Foster SJ, Williamson MP.
- Molecular basis for bacterial peptidoglycan recognition by LysM domains.
- Nature Communications, 5: 4269. doi: 10.1038/ncomms5269.
-
- Lowry RC, Parker JL, Kumbhar R, Mesnage S, Shaw JG, Stafford GP.
- The Aeromonas caviae AHA0618 gene modulates cell length and influences swimming and swarming motility.
- Microbiologyopen, 4: 220-234. doi: 10.1002/mbo3.233.
- 2013
- Prajsnar TK, Renshaw SA, Ogryzko NV, Foster SJ, Serror P, Mesnage S.
- Zebrafish as a novel vertebrate model to dissect enterococcal pathogenesis.
- Infection and Immunity, 81: 4271-4279. doi: 10.1128/IAI.00976-13
-
- Veyrier FJ, Williams AH, Mesnage S, Schmitt C, Taha MK, Boneca IG.
- De-O-acetylation of peptidoglycan regulates glycan chain extension and affects in vivo survival of Neisseria meningitidis.
- Molecular Microbiology, 87: 1100-1112. doi: 10.1111/mmi.12153
- 2012
- Benachour A, Ladjouzi R, Le Jeune A, Hébert L, Thorpe S, Courtin P, Chapot-Chartier MP, Foster SJ, Mesnage S.
- The peptidoglycan N-acetylglucosamine deacetylase PgdA (EF1843), produced upon exposure to lysozyme, contributes to Enterococcus
faecalis virulence. - Journal of Bacteriology, 194: 6066-6073. doi: 10.1128/jb.00981-12.
- 2011
- Wheeler R*, Mesnage S*, Boneca IG, Hobbs JK, Foster SJ.
- Super-resolution microscopy reveals cell wall dynamics and peptidoglycan architecture in ovococcal bacteria.
- Molecular Microbiology, 82: 1096-1109. doi: 10.1111/j.1365-2958.2011.07871.x.
- (* joint first-author)
- Dubée V, Chau F, Arthur M, Garry L, Benadda S, Mesnage S, Lefort A, Fantin B.
- The in vitro contribution of autolysins to bacterial killing elicited by amoxicillin increases with inoculum size in Enterococcus faecalis.
- Antimicrobial Agents and Chemotherapy, 55: 910-912. doi: 10.1128/AAC.01230-10.
- 2010
- Reste de Roca F, Duché C, Dong S, Rincé A, Dubost L, Pritchard DG, Baker JR, Arthur M, Mesnage S.
- Cleavage specificity of Enterococcus faecalis EnpA (EF1473), a peptidoglycan endopeptidase related to the LytM/lysostaphin family of
metallopeptidases. - Journal of Molecular Biology, 398: 507-17. doi: 10.1016/j.jmb.2010.03.033.
- 2009
- Emirian A, Fromentin S, Eckert C, Chau F, Dubost L, Delepierre M, Gutmann L, Arthur M, Mesnage S.
- Impact of peptidoglycan O-acetylation on the Enterococcus faecalis N-acetylglucosaminidase AtlA and N-acetylmuramidase AtlB.
- FEBS Letters, 583: 3033-3038. doi: 10.1016/j.febslet.2009.08.010.
- Bravetti AL, Mesnage S, Lefort A, Chau F, Eckert C, Garry L, Arthur M, Fantin B.
- Contribution of the AtlA autolysin to the bactericidal activity of amoxicillin against Enterococcus faecalis JH2-2.
- Antimicrobial Agents and Chemotherapy, 53: 1667-1669. doi: 10.1128/AAC.00692-08.
- 2008
- Mesnage S, Chau F, Dubost L, Arthur M.
- Role of N-acetylglucosaminidase and N-acetylmuramidase activities in Enterococcus faecalis PG metabolism.
- Journal of Biological Chemistry, 283: 19845-19853. doi: 10.1074/jbc.M802323200
- 2007
- Villet R, Fonvielle M, Busca P, Chemama M, Maillard AP, Hugonnet JEH, Dubost L, Marie A, Josseaume N, Mesnage S, Mayer C, Valéry JM, Ethève-Quelquejeu M, Arthur M.
- Idiosyncratic features in tRNAs participating in bacterial cell wall synthesis.
- Nucleic Acids Research, 35: 6870-6883. doi: 10.1093/nar/gkm778.
- Eckert C, Magnet S, Mesnage S.
- The Enterococcus hirae Mur-2 enzyme displays N-acetylglucosaminidase activity.
- FEBS Letters, 581: 693-696. doi: 10.1016/j.febslet.2007.01.033.
- 2006
- Eckert C, Lecerf M, Dubost L, Arthur M, Mesnage S.
- Functional analysis of AtlA, the major N-acetylglucosaminidase of Enterococcus faecalis.
- Journal of Bacteriology, 188: 8513-8519.
- 2005
- Maillard AP, Biarrotte-Sorin S, Villet R, Mesnage S, Bouhss A, Sougakoff W, Mayer C, Arthur M.
- Structure-based site-directed mutagenesis of the UDP-MurNAc-Pentapeptide-binding cavity of the FemX alanyl transferase from Weissella
viridescens. - Journal of Bacteriology, 187: 3833-3838.
- 2004
- Mignot T, Couture-Tosi E, Mesnage S, Mock M, Fouet A.
- In vivo Bacillus anthracis gene expression requires PagR as an intermediate effector of the AtxA signalling cascade.
- International Journal of Medical Microbiology, 293: 619-624.
- 2002
- Mignot T, Mesnage S, Tosi-Couture E, Mock M, Fouet A.
- Developmental switch of S-layer synthesis in Bacillus anthracis
- Molecular Microbiology, 43: 1615-1627.
- Couture-Tosi E, Delacroix H, Mignot T, Mesnage S, Chami M, Fouet A, Mosser G.
- Structural analysis and evidence for dynamic emergence of Bacillus anthracis S-layer networks.
- Journal of Bacteriology, 184: 6448-6456.
- Mesnage S, Fouet A.
- A plasmid-encoded autolysin in Bacillus anthracis: modular structure and catalytic properties.
- Journal of Bacteriology, 184: 331-334.
- 2001
- Mesnage S, Haustant M, Fouet A.
- A general strategy for identification of S-layer genes in the Bacillus cereus group: molecular characterization of such a gene in Bacillus
thuringiensis subsp. galleriae NRRL 4045. - Microbiology, 147: 1343-1351.
- 2000
- Mesnage S, Fontaine T, Mignot T, Delepierre M, Mock M, Fouet A.
- Bacterial SLH domain proteins are non-covalently anchored to the cell surface via a conserved mechanism involving wall polysaccharide
pyruvylation. - The EMBO Journal, 19: 4473-4484.
- 1999
- Mesnage S, Weber-Levy M, Haustant M, Mock M, Fouet A.
- Cell surface-exposed tetanus toxin fragment C produced by recombinant Bacillus anthracis protects against tetanus toxin.
- Infection and Immunity, 67: 4847-4850.
- Mesnage S, Tosi-Couture E, Fouet A.
- Production and cell surface anchoring of functional fusions between the SLH motifs of the Bacillus anthracis S-layer proteins and the Bacillus
subtilis levansucrase. - Molecular Microbiology, 31: 927-936.
- Mesnage S, Tosi-Couture E, Mock M, Fouet A.
- The S-layer homology domain as a means for anchoring heterologous proteins on the cell surface of Bacillus anthracis.
- Journal of Applied Microbiology, 87: 256-260.
- 1998
- Mesnage S, Tosi-Couture E, Gounon P, Mock M, Fouet A.
- The capsule and S-layer: two independent and yet compatible macromolecular structures in Bacillus anthracis.
- Journal of Bacteriology, 180:52-58.
- 1997
- Mesnage S, Tosi-Couture E, Mock M, Gounon P, Fouet A.
- Molecular characterization of the Bacillus anthracis main S-layer component: evidence that it is the major cell-associated antigen.
- Molecular Microbiology, 23: 1147-1155.